(Whole Blood/Serum/Plasma) indicatorSAMPLE COLLECTION AND STORAGE1. Collect Whole Blood /Serum / Plasma specimens following regular procedures. 2. Storage: Whole Blood can not be frozen. A specimen should be refrigerated if not used the same day of collection. Specimens should be frozen if not used within 3 days of collecting. Avoid freezing and thawing the specimens more than 2-3 times before using. 0.1% of Sodium Azide can be added to specimen as a preservative without affecting the results of the assay. ASSAY PROCEDURE 1. Using the enclosed plastic dropper for the sample, dispense 1 drop (10μl) of Whole Blood /Serum / Plasma to the circular sample well of the test card 2. Add 3 drops of Sample Diluent to the sample well, immediately after the specimen is added, from the dropper tip diluent vial (or all contents from the single test ampule). Interpret test results at 15 minutesREADING THE TEST RESULTS 1. Positive: Both a purplish red test band and a purplishred control band appear on the membrane. The lower the antibody concentration, the weaker the test band. 2. Negative: Only the purplish red control band appears on the membrane. The absence of a test band indicates a negative result. 3. Invalid result: There should always be a purplishred control band in the control region, regardless of the test result. If a control band is not seen, the test is consider re-invalid. Repeat the test using a new test device. Note: It is normal to have a slightly lightened control band with very strong positive samples, as long as it is distinctly visible.