Volume 122 of Methods in Cell Biology describes modern tools and techniques used to study nuclear pore complexes and nucleocytoplasmic transport in diverse eukaryotic model systems ‎(‎including mammalian cells‎,‎ Xenopus‎,‎ C‎.‎ elegans‎,‎ yeast‎)‎‎.‎ The volume enables investigators to analyze nuclear pore complex structure‎,‎ assembly‎,‎ and dynamics‎;‎ to evaluate protein and RNA trafficking through the nuclear envelope‎;‎ and to design in vivo or in vitro assays appropriate to their research needs‎.‎ Beyond the study of nuclear pores and transport as such‎,‎ these protocols will also be helpful to scientists characterizing gene regulation‎,‎ signal transduction‎,‎ cell cycle‎,‎ viral infections‎,‎ or aging‎.‎ The NPC being one of the largest multiprotein complexes in the cell‎,‎ some protocols will also be of interest for people currently characterizing other macromolecular assemblies‎.‎ This book is thus designed for laboratory use by graduate students‎,‎ technicians‎,‎ and researchers in many molecular and cellular disciplines‎.‎ * Describes modern tools and techniques used to study nuclear pore complexes and nucleocytoplasmic transport in diverse eukaryotic model systems ‎(‎mammalian cells‎,‎ Xenopus‎,‎ C‎.‎ elegans‎,‎ yeast‎)‎* Chapters are written by experts in the field * Cutting‎-edge material