Approaches to Research on the Systematics of Fish‎-Borne Trematodes is a concise guide for systematic studies of the prevalence of fish‎-borne trematodes both in the endemic areas and experimental laboratories‎.‎ It includes methods to identify species of fish‎-borne trematodes to enhance the precision of research studies based on the metacercarial stage‎.‎ Misidentification of trematode species is a common occurrence when researchers are new to the field and have no guidance‎.‎ Consequentially‎,‎ sometimes publications report inaccurate prevalence rates of these parasites‎.‎ This compact guide gives clear direction on‎:‎ * Collection of parasites in the final hosts* Collection of cercaria from snail first intermediate hosts* Collection of metacercaria from fish hosts* Molecular identification of parasites* Systematics of fish‎-borne trematodes * Provides research guidelines and protocols for studying systematics of fish‎-borne trematodes using both morphological and molecular data* Presents keys to enable identification of metacercariae of fish‎-borne trematodes in the Greater Mekong subregion